For the pulmonary samples, the sensitivity, specificity, and positive and negative predictive values were For microscopically negative specimens, the respective values were The assay correctly detected rifampin resistance in all but one specimen, which harbored a mixed population.
The GeneXpert assay was highly effective for tuberculosis diagnosis and identification of rifampin-resistant strains in smear-negative samples. Tuberculosis TB remains a major public health problem,
Genexpert mtb fdating for more than 9.
In order to overcome conventional methods' low sensitivity and diagnostic delays, nucleic acid amplification NAA tests have been introduced. The identification of mutations associated with drug resistance depends on additional NAA tests, whose application on clinical samples is indicated only for AFB-positive specimens.
The test is carried out within 2 h in a disposable cartridge. The only manual step is the
Genexpert mtb fdating of a bactericidal
Genexpert mtb fdating with the sample prior to addition to the cartridge. This preamplification step reduces the viability of MTBC organisms, making the assay suitable for use near patients in settings with limited biocontainment facilities 1.
Specimens were selected from patients with strong clinical indications for TB. A small number of AFB-positive specimens
Genexpert mtb fdating also included to serve as positive controls.
Specimens were processed by the standard N -acetyl- l -cysteine and sodium hydroxide method. A smear of the processed sediment was prepared and examined for the presence of AFB. The specimens analyzed
Genexpert mtb fdating the culture results are presented in Table 1 samples, 80 pulmonary and 41 extrapulmonary, from patients; AFB negative, 1 suspicious, and 15 AFB positive.
Culture data were available for samples. One hundred nineteen samples Thirty-eight were positive and 81 negative. Only 1 of the 6 had a subsequent positive culture that was taken into consideration in the final analysis.
Furthermore, in one additional case, a complex heteroresistant pattern was observed, as the patient's sample harbored strains with either the HL or the SL mutation. Informative for both assays were 32 samples Table 3. Among samples, 35 were positive for MTBC.
An additional AFB-negative sample exhibiting the same pattern came from a patient proven to have pulmonary TB. The higher sensitivity for AFB-negative specimens was attained upon testing of additional samples that increased the initial value from We performed one or
Genexpert mtb fdating additional tests for 11 AFB-negative cases. Discrepancies between the first Xpert-negative and
Genexpert mtb fdating second Xpert-positive sample were detected in two cases.
All cultures from consecutive specimens of one of these patients were negative. In contrast, cultures from the second patient's consecutive sputa were both positive for an MDR M. This case of MDR M. Five of the 35 MTBC culture-positive samples showed rifampin resistance. The strain incorrectly identified as rifampin sensitive derived from a patient with a mixed heteroresistant population.
However, and despite the presence of a mixed population, a delay in probe hybridization was also observed upon sample processing, but it was not sufficient for the correct interpretation.
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Since the assay does not detect specific mutations but deviations from the wild-type sequence, it is expected that in certain cases, i. With the exception of a specimen harboring a mixed MTBC population, the assay correctly retrieved information from the rpoB hot spot region regarding RMP resistance. Accumulating published
Genexpert mtb fdating, including those presented herein, indicate that this rapid and easy-to-perform fully automated NAA test could prove to "Genexpert mtb fdating" an extremely helpful diagnostic tool in the fight against tuberculosis.
National Center for Biotechnology InformationU.
evaluation of the GeneXpert® MTB/RIF...
Journal List J Clin Microbiol v. Author information Article notes Copyright and License information Disclaimer. This article has been cited by other articles in PMC.
Specimens analyzed and culture results. AFB result and source of specimen s No. Open in a separate window. Rapid molecular detection of tuberculosis and rifampin resistance.
Centers for Disease Control Prevention Updated guidelines for the use of nucleic acid amplification
Genexpert mtb fdating in the diagnosis of tuberculosis. Rapid detection of Mycobacterium tuberculosis and rifampin resistance by use of on-demand, near-patient technology. Rapid detection of Mycobacterium tuberculosis complex and rifampin resistance in smear-negative clinical samples by use of an integrated real-time PCR method. Comparison of three molecular assays for rapid detection of rifampin resistance in Mycobacterium tuberculosis.
The GeneXpert MTB/RIF assay was evaluated with microscopically negative and positive pulmonary and extrapulmonary specimens from patients with.
She knows I like her? Accuracy and impact of Xpert MTB/RIF for the diagnosis of smear-negative or sputum-scarce tuberculosis using bronchoalveolar lavage fluid. Free. Loading. evaluation of the GeneXpert® MTB/RIF assay for the rapid detection of human infected with TB, Egypt has documented TB dating back to 5, years ago..
For the pulmonary samples, the sensitivity, specificity, and positive and negative predictive values were For microscopically negative specimens, the respective values were The assay correctly detected rifampin resistance in all but one specimen, which harbored a mixed population. The GeneXpert assay was highly effective for tuberculosis diagnosis and identification of rifampin-resistant strains in smear-negative samples.
Tuberculosis TB remains a major public health problem, accounting for more than 9. In order to overcome conventional methods' low sensitivity and diagnostic delays, nucleic acid amplification NAA tests have been introduced. The identification of mutations associated with drug resistance depends on additional NAA tests, whose application on clinical samples is indicated only for AFB-positive specimens.
The test is carried out within 2 h in a disposable cartridge. The only manual step is the mixing of a bactericidal buffer with the sample prior to addition to the cartridge.
In comparison, standard cultures can take 2 to 6 weeks for MTBC to grow and conventional treat resistance tests can augment 3 more weeks. Tuberculosis is one of the deadliest public health threats today.
Traditionally, tuberculosis is mostly being diagnosed by way of a combination of case X-rays, the staining of sputum with special dyes followed by microscopy, the growth of Mycobacterium tuberculosis in culture and the Mantoux test.
The sputum smear microscopy is painless to do and is very cheap and combined with chest X-rays has been used for a long time by TB control agencies worldwide. Despite that the sputum smear microscopy sputum AFB test has some problems in HIV-positive patients and children, as well as patients with low bacterial load. The determination of drug susceptibility is particularly relevant as Mycobacterium tuberculosis becomes increasingly resistant to two of the major anti-tuberculosis drugs, isoniazide and rifampicin.
That form of tuberculosis is called multi-drug-resistant tuberculosis MDR-TB is rapidly on the rise globally.
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Rapid detection of Mycobacterium tuberculosis and rifampin resistance by use of on-demand, near-patient technology. Rapid detection of Mycobacterium tuberculosis complex and rifampin resistance in smear-negative clinical samples by use of an integrated real-time PCR method.
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